The medium is also the most important factor for the culturing of cells. There are different medium options that researchers can use to culture cells. The medium that is used for cell culture has to be free of all contaminants in order for the quality of the cells to be consistent and reliable. However, the culture medium can be contaminated with pathogens such as bacteria, viruses, and fungi, which can contaminate the cells and influence cell growth. Researchers should therefore test the medium and water before purchasing it from commercial vendors.
The most common cell culture medium is L-15, the medium that is used to culture cells in most laboratories. However, for the culture of cells in mammalian cells in vitro, there are some essential factors that are missing in L-15, including serum, transferrin, insulin, and thyroid hormone. Researchers have used other media to supplement L-15. These media include Dulbecco’s Modified Eagle’s Medium (DMEM), BIOAMINE® DMEM (B-DMEM), DMEM-F12, Ham’s F12, Roswell Park Memorial Institute (RPMI), and N2A, among others.
Studies have shown that L-15 is an effective media for the culture of cells in vitro. However, the L-15 medium had some drawbacks, for instance, the medium has no ability to support the growth of cells. In order to solve this issue, the researchers introduced a medium that was developed by Life Technologies Corporation in 1996. A group of researchers at the company developed a medium called Nutrient Mixture F12 (Nu-M11) that was designed to support the growth of mammalian cells in vitro. The medium also contains several hormones and components, including serum, transferrin, insulin, and thyroid hormone. Compared with L-15, Nu-M11 was reported to be more effective than L-15 for cell culture.
The most important property of a cell culture medium is the proliferation of the cells, which results in more cells, more proteins, and more data. Secondly, the medium must maintain cell viability and metabolism for a period of time, ensuring that cellular metabolism and function are not significantly disturbed. The medium must also provide nutrients for growth and support proper cellular functions.
Likewise, the growth in cells cultured in media reconstituted with UF water also was higher when cells were cultured in spinner flasks. For cells in spinner flasks cultured in UF water-reconstituted media, the mean cell density was 2.70 x 106 cells/mL, and an average viability of 97.3% was reached (Figure 3B). In comparison with these results, a mean cell density yield of 2.34 x 106 cells/mL and a viability of 97.32% were achieved in the cells cultured in media reconstituted in RO water.
Other factors, such as the culture medium, physical conditions of the flasks and the cells themselves, should also be taken into consideration when optimizing the culture conditions of cells cultured in spinner flasks.
These results showed that the growth of cells cultured in media reconstituted with UF water in spinner flasks was substantially higher than the growth of cells cultured in media reconstituted with RO water. 827ec27edc