Finally, we examined the evolution of the 33 cell types from four species (humans, mouse, marmoset, and mouse). We first identified the homologous groups of the 33 cell types from four species by comparing them with the integrated tree by applying criteria in two steps. First, any node representing a species was recursively searched to find the node with the highest bootstrap value of the species. If the bootstrap value of the node was larger than 90%, it was considered as the node representing the homologous group of the 33 cell types. Any tree branches below this node (or the root node) were pruned, and the cells were merged into the homologous group based on the integrated tree. For example, the corresponding bootstrap values of the human, mouse, marmoset, and mouse were 93, 99, 99, and 99, respectively. Because the bootstrap value of the human node was larger than 90%, we set the human node as the node representing the homologous group of the 33 cell types.
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